Test Kits to Test Hormones from Home
TEST! DON'T GUESS! Order Your Saliva Test Kit.
Home test, mail deliver. It will provide you an easy to read
and understand report and tells you which hormones replacement therapy
support creams you need.
Pre-Paid 2-Hormone Saliva
Home Collection Test Kit
Two Hormone Pre-paid lab tests with this Home Collection Kit
and ZRT Lab
select the 2 hormones. One tube, specimen collected in AM.
Women usually select Estradiol (E2) & Progesterone (Pg). Men usually
select Estradiol (E2) and Testosterone (T).
You may buy additional kits and test for any 2 hormones of your choice
with each kit.
Panel III - Saliva Home Collection Test Kit
Eight Hormone Pre-paid lab tests with this Home Collection Kit
"The Works"- Use for Hormone Panel III with ZRT Lab
Four tubes: collected 4 times during day, morning, noon, afternoon,
Best comprehensive assessment of
adrenal cortisol stress hormones and total sex hormone function;
recommended with excessive stress, chronic fatigue, sleep disorders and
allergies. Most Recommended for men and women. "The works" in
Hormone Panel III provides the most complete and in-depth evaluation
of hormone balance, imbalance and interrelationship of your specific
hormone levels as related to symptoms of Menopause, Perimenopause,
Pre-menopause, Post-menopause, Andropause and Adrenal
function. Eight Tests: Estradiol (E2), Progesterone (Pg), Testosterone
(T), DHEA (DS) and all four Cortisol adrenal stress levels
throughout the day (C1, C2, C3, C4).
All instructions are included for
obtaining the saliva sample. You do not need to stop hormone
treatments to test saliva. Instructions included. Collect
specimens all in same day. The saliva
specimen is sent BY YOU to ZRT
Laboratory in a convenient mailer, and results are confidentially returned
TO YOU with interpretations. NOTE: The ORIGINAL of
the Hormone Prepaid Requisition Form, marked on lower right of Form
inside each kit, MUST be sent to ZRT to obtain credit for your
pre-payment of the lab testing fees.
If you do not submit the pre-paid forms, we will not refund and ZRT has
no way to know you are prepaid. Please follow all kit instructions
carefully inside the kit. Testing done
confidentially by ZRT Labs. Testing is not done by HormoneProfile.com
California Residents ONLY:
California State health law
requires that the testing of any specimen collected or
mailed from California be sent with a written order from
a health care professional licensed in California to
order laboratory tests. This includes the following
disciplines: M.D.; D.C.; LAc; R.D.; D.O.; N.P.; and
Pharmacists (R.PH). As of September 2002 (Senate Bill
577), such lab tests may be ordered by complementary or
alternative health care practitioners "not providing
services that require medical training."
for Maryland and New York Residents ONLY:
Maryland and New York State health law
prohibits the testing of specimens mailed from or to
Maryland or New York addresses, physicians, or residents.
Therefore, direct receipt of lab results for NY
residents is not possible. Maryland and New York
private residents have been using the address of someone in
other states are effected. We can sell and ship kits to you in New
York and California, but YOU MUST comply with the above. We will not
refund, nor can ZRT. YOU must comply.
Saliva Testing Frequently Asked Questions
HormoneProfile.com connected to ZRT Laboratory? No.
Hormone Profile.com sells the saliva hormone test kits because
we believe it is the best laboratory in the country. We
recommend Dr David Zava and his lab because it was he who INVENTED the
saliva test for bioavailable hormones and he has a life long commitment
to hormone health for men and women. We respect that. We do
not see your results. We do not test your results.
That is done by the top clinical professionals at ZRT
laboratory. Once you have your saliva test kit, we are out
of the picture, until you are ready to order the hormone
treatment products recommended from your testing.
Can Saliva Hormone Testing Help You?
testing can identify hormone imbalances that may be causing
unwanted symptoms and chronic health problems... like
fatigue, low sex drive, hot flashes, foggy thinking, stress,
mood swings, sleep disturbances, anxiety, depression...
can be used to monitor the effect of hormone treatment
is vital for disease prevention and anti-aging programs
will tell you your best course of treatment
testing is the most reliable way to measure free,
"bio-available" hormone activity - hormones
actually doing their job at the cell level. Most
blood tests do not measure "bio-available" hormone
hormones more accurately reflect tissue uptake and response
of hormones delivered through the skin in creams, gels, or
patches. Blood and urine assays significantly
underestimate hormones delivered topically, often resulting
collection is painless - no needles. It negates the
need for blood drawing, which often causes stress that can
alter test results.
collection is easy and can be done anyplace at your
Why Test Saliva Instead of Serum (Blood
Tests for Hormones)
WHY TEST SALIVA instead of
SERUM (Blood tests)
SPECIAL REPORT Dr David Zava, ZRT
Confusion exists among medical
professionals and the general public about the question of
progesterone absorption. This confusion often hinges on a
misunderstanding of the test used to measure progesterone
levels in the body. Let us try to clarify the issue.
What a Blood Test Measures
"Blood" tests for
progesterone refer to the serum or plasma concentration of
progesterone. Plasma is the watery, non-cellular portion of
the blood from which cellular components such as red blood
cells and white blood cells, are excluded. Serum is the
essentially the same as plasma except that fibrinogen has been
removed. Serum and plasma, being watery, contain water-soluble
(hydrophilic) substances such as water-soluble vitamins,
carbohydrates, and proteins. Serum and plasma do not contain
fat-soluble (lipophilic) substances. For the purposes of this
discussion, serum and plasma are interchangeable and I will
refer to them as serum. Sex hormones such as progesterone,
estrogen and testosterone are fat-soluble steroids similar to
cholesterol. When you have a serum cholesterol measurement,
you are measuring cholesterol bound to protein, which makes it
water-soluble. (Recall that serum cholesterol is described as
HDL or LDL cholesterol, referring to the proteins to which it
Progesterone Travels in Blood
The ovary-produced progesterone
found in serum is also largely protein-bound. Protein-bound
progesterone is not readily bioavailable to receptors in
target tissues throughout the body. It is on its way to the
liver to be excreted in bile. Only 2 to 5 percent of serum
progesterone is "free" or non-protein-bound. This is
the progesterone available to target tissues and to saliva.
Thus, progesterone measured by serum levels is mostly a
measure of progesterone that is not going to be used by the
body. A serum test can be used to compare one woman's
progesterone production to that of another woman, or to test
how much progesterone is being made by a woman's ovaries.
When progesterone is given
intravenously, 80 percent of it is taken up by red blood cell
membranes that are fatty in nature and therefore available to
fat-soluble progesterone molecules. Less than 20 percent will
be found in serum. It is obvious that serum levels would not
detect the great majority of the progesterone added to whole
of Transdermal Progesterone
Progesterone is a highly
lipophilic (fat loving) molecule that is well absorbed through
skin into the underlying fat layer. In fact, it is among the
most lipophilic of the steroid hormones. From the fat layer,
the progesterone is taken up gradually by red blood cell
membranes in capillaries passing through the fat. The
progesterone transported by red blood cell membranes is
readily available to all target tissues and to saliva. This
progesterone is completely bioavailable and readily measured
by saliva testing. Only a small fraction of it is carried by
the watery serum. Obviously, serum testing is not a good way
to measure transdermal progesterone absorption.
Yet, many doctors continue to
question the skin absorption of progesterone. A recent example
is a report in the
April 25, 1998 issue of the Lancet that
serum levels did not reflect a substantial rise of
progesterone after topical application in postmenopausal
women. This report is being used to argue that progesterone is
not well absorbed. This implication is erroneous. Rather, it
means that the authors did not understand the significant
difference between serum and saliva progesterone levels. Some
even imply that saliva testing is relatively unknown and its
reliability is unproven. This is an odd admission since
researchers have been using saliva testing for years and a
number of laboratories offer routine saliva hormone testing. A
sampling of references supporting all points of importance in
this matter can be found at the end of this report.
HOW TO USE
SALIVA HORMONE ASSAY TO DETERMINE PROGESTERONE DOSAGE
Achieving Balance is the Key
The goal of progesterone
supplementation is to restore normal physiologic levels of
bioavailable progesterone. Progesterone/estrogen balance is
the key. When sufficient numbers of normal ovulating women are
tested by saliva hormone assay, the typical range of
progesterone is found to be 0.3 to 0.5 ng/ml. Under usual
circumstances, there should be no reason to exceed that range.
In my experience, the topical
dose required to achieve a saliva level of 0.5 ng/ml is
commonly only 12 to 15 mg per day. For creams containing 900
to 1000 mg per 2-oz container, 12-15 mg a day for 24 days
would use up only about one-third of a 2-oz container. Larger
doses are often used initially to "catch up" on the
existing progesterone deficiency state, but the maintenance
dose will usually be around 15 mg per day. Since considerable
variation in progesterone is well tolerated, a modest
elevation of saliva levels to 0.8 to 1.5 ng/ml is acceptable.
Levels and PMS
Saliva progesterone levels
several times higher than 0.5 ng/ml are justified in certain
situations. In PMS, for example, stress is often a factor.
Stress increases cortisol production. Cortisol blockades some
progesterone receptors and thereby prevents progesterone
function. To compete with this cortisol blockade, topical
progesterone in the range of 30 to 40 mg/day is sometimes
initially required to achieve a beneficial effect.
Likewise, in women with
endometriosis, the goal is to increase progesterone levels to
that found in women two months pregnant. This level may
require that supplemental topical progesterone be in a range
of 30 to 50 mg/day from day 8 to day 26 of the menstrual
cycle. (See the July 98 issue of the John Lee Medical Letter,
for a more detailed article on the causes and treatment of
Progesterone dosage is
determined largely by response: the right dose is the amount
that results in progressive decrease of endometriosis pain.
When pain is largely gone, levels can be decreased gradually
over time to doses necessary to maintain the progesterone
and Estrogen Receptors
In women whose doctors are
giving them excessive supplemental estrogen, a different
problem must be faced. Excessive estrogen in circumstances of
deficient progesterone induces a decrease in receptor
sensitivity. One of progesterone's functions is to restore the
normal sensitivity of estrogen receptors. When progesterone is
restored, estrogen receptor sensitivity is restored, also. It
is not surprising that, in these cases, some women develop
symptoms of estrogen dominance (water retention, headaches,
weight gain, swollen breasts) when progesterone is first
supplemented. Obviously, the estrogen dose must be lowered. If
this is done too rapidly, however, hot flushes can occur. The
key is to reduce estrogen gradually while progesterone is
In my experience, estrogen
dosage can be reduced 50 percent as soon as progesterone is
added. Then, every 2 to 3 months, the estrogen dose can be
further decreased gradually. Many women eventually discover
they do not need any supplemental estrogen at all: the
estrogen normally produced by body fat in postmenopausal women
is often sufficient for its needs once the progesterone is
Saliva Hormone Testing as Used by Researchers
Painter-Brick C, Lotstein DS,
Ellison PT. Seasonality of reproductive function and weight
loss in rural Nepali women. Hum Reprod May 1993; 8 (5):
Ellison PT, Painter-Brick C,
Lipson SF, O'Rourke MT. The ecological context of human
ovarian function. Hum Reprod Dec 1993; 8 (12): 2248-2258.
Ellison PT. Measurements of salivary progesterone. Ann NY Acad
Sci Sept 20 1993; 694: 161-176.
Campbell BC, Ellison PT.
Menstrual variation in salivary testosterone among regularly
cycling women. Horm Res 1992; 37 (4-5): 132-136.
Lipson SF, Ellison PT.
Reference values for luteal "progesterone" measured
by salivary radioimmunoassay. Fertility and Sterility May
1994; 61 (3): 448-454.
Bloom T, Ojanotko-Harri A,
Laine M, Huhtaniemi I. Metabolism of progesterone and
testosterone in human parotid and submandiblular salivary
glands in vitro. J Steroid Biochem Mol Biol Jan 1993; 44 (1):
Good Evidence Concerning the
Absorption of Steroids Through Human Skin Johnson ME, et al.
Permeation of steroids through human skin. J Pharmaceutical
Sci 1995; 84: 1144-1146.
The Evidence of Red Blood Cell
Transport of Progesterone Devenuto F, et al. Human erythrocyte
membrane: Uptake of progesterone and chemical alterations.
Biochim Biophys Acta, 1969;193:36-47. Koefoed P, Brahm J.
Permeability of human red cell membrane to steroid sex
hormones. Biochim Biophys Acta 1994; 1195: 55-62.
Direct Comparison of Plasma and
Saliva Levels After Topical Progesterone Application Dollbaum
CM, Duwe GF. Absorption of progesterone after topical
application: plasma and saliva levels. Presented at the 7th
Annual Meeting of the American Menopause Society, 1997.
The last reference is
particularly revealing. Creams with varying concentrations of
progesterone were applied to menopausal women after which both
plasma and saliva levels were measure. The results are
0.36 + 0.06
0.50 + 0.09
1.8 + 0.3
0.03 + 0.006
0.152 + 0.025
8.7 + 3.5
As can be seen, in these menopausal women given the placebo
topical cream, the plasma level was more than 10 times greater
than saliva level. This indicates how little of their blood
progesterone was of the non-protein-bound, bioavailable kind.
When only 0.34 mg of
progesterone was applied topically, the plasma level rose 39
percent, whereas saliva level rose 5-fold. This indicates that
only a small portion of the added progesterone entered the
plasma, whereas the saliva clearly showed a hefty increase of
bioavailable progesterone. When an 88-fold larger dose was
applied topically, the plasma level rose only 3.6-fold while
the simultaneous saliva level rose 57-fold. This indicates
that only the saliva reflected the great increase in absorbed
bioavailable progesterone. The progesterone found in the
saliva obviously was blood-borne, but it should be clear that
the portion of the blood carrying the progesterone was not the
plasma (serum) but, rather, was via red blood cells.
In all situations, however, it
should be clear that plasma progesterone levels are not
indicative of the true level of bioavailable progesterone such
as is obtained from topical application. Saliva levels are far
more appropriate for this purpose
test results and patient questionnaires make the ZRT method unique, said
Zava, who co-authored the book "What Your Doctor May Not Tell You About
Breast Cancer: How Hormone Balance Can Help Save Your Life." "We don't
diagnose, but the test results are so comprehensive they give doctors a
tool to work with," Burch said. The results can be used to design unique
hormone treatments. The traditional one-size-fits-all approach to
hormone therapy is inadequate, Zava said. "It needs to be customized for
each person because everyone's needs are different," Zava said
ZRT Laboratory favors
the use of bioidentical hormones, identical in structure and function to
those produced naturally by the body, in place of the synthetic ones
traditionally used in hormone replacement therapy. Compounding pharmacists
can tailor the dose and the delivery of natural hormone supplements to
suit individuals. Hormones influence mood, the energy level and sleep
patterns, among other functions. If unbalanced, they can wreak havoc on
the body, prompting endometriosis, hair loss, low libido, fatigue,
fibroids and infertility, in addition to the persistent symptoms of
menopause and andropause, including hot flashes, mood swings and weight
HIGHLIGHTS of the RESEARCH STUDIES
Women's Health Initiative & National Institutes Health
Serious Health Risks of the Prescription Synthetic
Women's Health Initiative WHI Study site:
New England Journal of Medicine:
Journal of the American Medical Association:
National Institutes of Health:
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